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33:49
YouTube
the bumbling biochemist
Determining concentration & purity of RNA & DNA with UV spectroscopy: Beer's Law and Beyond!
We can use the absorption at a single wavelength to calculate the concentration and look at multiple wavelengths to get info on purity. Because molecules have overlapping spectra you look to ratios. A couple key ratios for when you’re analyzing nucleic acid (DNA or RNA) quality are 260/230 & 260/280. full text & figs: http://bit.ly/dnauvbeer ...
10.1K views
Oct 28, 2021
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